Visualization and Quantification of Transposon Activity in Caenorhabditis elegans RNAi Pathway Mutants
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چکیده
منابع مشابه
Natural and Unanticipated Modifiers of RNAi Activity in Caenorhabditis elegans
Organisms used as model genomics systems are maintained as isogenic strains, yet evidence of sequence differences between independently maintained wild-type stocks has been substantiated by whole-genome resequencing data and strain-specific phenotypes. Sequence differences may arise from replication errors, transposon mobilization, meiotic gene conversion, or environmental or chemical assault o...
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A second family of transposons, named Tc2 elements, has been identified in the nematode Caenorhabditis elegans. Tc2 elements are polymorphic in sequence and are present in different numbers in different strains. Like the transposon Tc1, Tc2 is active in the germ line of some C. elegans strains. A high rate of transposition has been observed in the progeny of certain interstrain crosses, where t...
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Gene silencing mediated by dsRNA (RNAi) can persist for multiple generations in Caenorhabditis elegans (termed RNAi inheritance). Here we describe the results of a forward genetic screen in C. elegans that has identified six factors required for RNAi inheritance: GLH-1/VASA, PUP-1/CDE-1, MORC-1, SET-32, and two novel nematode-specific factors that we term here (heritable RNAi defective) HRDE-2 ...
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The metaphase to anaphase transition is a critical stage of the eukaryotic cell cycle, and, thus, it is highly regulated. Errors during this transition can lead to chromosome segregation defects and death of the organism. In genetic screens for temperature-sensitive maternal effect embryonic lethal (Mel) mutants, we have identified 32 mutants in the nematode Caenorhabditis elegans in which fert...
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The mevalonate pathway is highly conserved and mediates the production of isoprenoids, which feed into biosynthetic pathways for sterols, dolichol, ubiquinone, heme, isopentenyl adenine, and prenylated proteins. We found that in Caenorhabditis elegans, the nonsterol biosynthetic outputs of the mevalonate pathway are required for the activity of microRNAs (miRNAs) in silencing their target mRNAs...
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ژورنال
عنوان ژورنال: G3 Genes|Genomes|Genetics
سال: 2019
ISSN: 2160-1836
DOI: 10.1534/g3.119.400639